Abstract:Rapid on-site evaluation is a cytological assessment technique for specimens obtained during endoscopic ultrasound-guided fine-needle aspiration/biopsy. It plays different roles in evaluating specimen adequacy, reducing repetitive procedures, and improving diagnostic efficiency. Currently, there is no guideline or consensus in China to guide clinical practice. Based on existing clinical evidence-based medicine, Digestive Endoscopy Branch of Chinese Medical Association, Gastroenterologist and Hepatologist Branch of Chinese Medical Doctor Association, National Clinical Research Center for Digestive Diseases and State Key Laboratory of Digestive Health organized relevant experts to discuss its definition and purpose, applicable scenarios, technical operation specifications, training for gastroenterologists, process of evaluation results, and alternative solutions. A consensus was reached, aiming to provide reference and guidance for the standardized implementation of rapid on-site evaluation of specimens obtained through endoscopic ultrasound‑guided fine-needle aspiration/biopsy and to promote the standardization and normalization of endoscopic ultrasound-guided puncture procedures.