Abstract:To evaluate the diagnostic value and safety of endoscopic ultrasound‑guided fine needle aspiration (EUS‑FNA) in patients with biopsy‑negative malignant gastrointestinal stricture, a retrospective analysis was performed on data of patients whose computed tomography scan showed gastrointestinal malignant tumor with complete lumen stricture and endoscopic biopsy results showed negative, who underwent EUS‑FNA in the Second Affiliated Hospital of Soochow University from July 2016 to January 2020. Perioperative complications, technical success rate and accuracy of EUS‑FNA were analyzed. Eleven cases included in the study, including 7 males and 4 females, with mean age of 60.3 years. There were 8 esophageal strictures and 3 rectal strictures. All patients successfully underwent EUS‑FNA, and malignant tumor was found in 10 cases and no tumor cell was found in 1 case. No complications were reported. EUS‑FNA is a safe and valuable approach to diagnosing biopsy‑negative malignant gastrointestinal strictures.