生物膜清洗剂与多酶清洗剂对内镜生物膜清除效果的对比研究

Comparison of biofilm cleaning agent and multi-enzyme detergent on endoscopic biofilm cleaning effects

摘要:

目的 探索生物膜清洗剂与2种多酶清洗剂在内镜生物膜清除效果上的差异。方法 建立铜绿假单胞菌内镜生物膜模型，对照组采用灭菌水浸泡，实验组分别采用1号多酶清洗剂、2号多酶清洗剂及生物膜清洗剂进行浸泡。在室温下分别作用5、10、15 min后，以细菌活菌计数法及扫描电镜法评价不同清洗剂对内镜生物膜模型的清洗效果。各组间活菌计数比较采用方差分析。结果 作用5、10、15 min后, 测定的生物膜菌落计数(CFU/cm2)空白对照组为7.92±0.21，1号多酶清洗剂组分别为5.31±0.10、5.04±0.08、4.90±0.16，2号多酶清洗剂组分别为5.53±0.30、5.39±0.21、5.03±0.42，生物膜清洗剂组分别为3.53±0.30、3.01±0.07、2.82±0.26。在相同作用时间下，两种多酶清洗剂组的菌落计数比较差异无统计学意义（P＞0.05），生物膜清洗剂组菌落计数少于两种多酶清洗剂组（P<0.05）。且随着浸泡时间增加，生物膜清洗剂组菌落计数逐渐减少（P＜0.05）。作用15 min后，扫描电镜下见生物膜清洗剂组清洗后的生物膜及细菌残留最少。结论 两种多酶清洗剂的清洗效果相当，生物膜清洗剂较多酶清洗剂对内镜生物膜的清除效果更好，可明显提高内镜清洗质量。

Abstract:

Objective To investigate the cleaning effects of biofilm cleaning agent and two kinds of multi-enzyme detergents on endoscopic biofilm. Methods Endoscopic biofilm model was established using pseudomonas aeruginosa, and soaked with No. 1 multi-enzyme detergent, No. 2 multi-enzyme detergent, and biofilm cleaning agent respectively. The control group was cleaned with sterile water. After 5, 10, and 15 minutes at room temperature, the cleaning effects were evaluated by bacteria counting method and scanning electron microscope. Arova was used for the comparison of viable counts among groups. Results At 5, 10, and 15 minutes of soak, the standard colony counts (CFU/cm2) of biofilm was 5.31±0.10, 5.04±0.08 and 4.90±0.16 in the No.1 multi-enzyme detergent group, 5.53±0.30, 5.39±0.21 and 5.03±0.42 in the No.2 multi-enzyme detergent group, and 3.53±0.30, 3.01±0.07 and 2.82±0.26 in the biofilm cleaning agent group, and 7.92±0.21 in the blank control group. There was no significant difference in the colony counts between the two multi-enzyme detergent groups (P＞0.05). However, the colony counts of biofilm cleaning agent group was less than that of the two multi-enzyme detergent groups (P<0.05), and decreased with time (P＜0.05). Under scanning electron microscope, the biofilm cleaning agent group had the least residual biofilm and bacteria. Conclusion Biofilm cleaning agent can significantly improve the quality of endoscopic cleaning, and is worthy of clinical promotion.