细针型共聚焦激光显微内镜在体成像的动物实验研究

Imaging of needle-based confocal laser endomicroscopy in rabbit models

摘要:

目的 探索细针型共聚焦激光显微内镜（nCLE）对动物腹腔组织及脏器成像的可行性。方法 选取实验家兔的大网膜、肝脏、胰腺、腰大肌（骨骼肌）为研究对象，nCLE经由19 G穿刺针进入其内部分别进行成像，观察各腹腔组织及脏器的成像特征，并留取清晰的nCLE图像。最终将nCLE图像与病理组织学结果相比对。结果 5只实验家兔腹腔组织及脏器的nCLE成像均成功完成，并清晰显示大网膜、肝脏、胰腺、腰大肌（骨骼肌）的细胞、腺体、微血管等显微结构，其nCLE图像具有特征性。各腹腔组织及脏器nCLE图像与病理组织学具有良好的一致性。结论 利用nCLE对腹腔组织及脏器成像具有可行性。

Abstract:

Objective To evaluate the feasibility of needle-based confocal laser endomicroscopy (nCLE) for imaging of intra-abdominal tissues and organs in rabbit models in vivo. Methods The nCLE miniprobe was inserted through the 19-gauge needle into various intra-abdominal tissues and organs [omentum majus, liver, pancreas and psoas major (skeletal muscle)]. The nCLE images were acquired and real-time sequences of respective locations were recorded. Finally, nCLE image characteristics were compared with histopathologic findings. Results nCLE was successfully performed in intra-abdominal tissues and organs of five rabbit models. The microscopic structure of cells, glands and microvessels in the omentum majus, liver, pancreas and psoas major (skeletal muscle) was visualized clearly, respectively. Characteristics of various intra-abdominal tissues and organs were displayed on nCLE images, which were correlated well with histological findings. Conclusion Imaging of intra-abdominal tissues and organs with nCLE in vivo is feasible in future clinical practice.